Essential sites at transposon Tn 10 termini.

Kivisaar and Rita Hõrak 4652 Transposon Tn putida Pseudomonas Target

Sequence analysis of termini of conjugative transposon Tn916.

Transposon Tn916 is a 16.4-kilobase, broad-host-range, conjugative transposon originally identified on the chromosome of Enterococcus (Streptococcus) faecalis DS16. Its termini have been sequenced along with the junction regions for two different insertions. The ends were found to contain imperfect inverted repeat sequences with identity at 20 of 26 nucleotides. Further in from the ends, imperf...

Reduced expression of Tn 10-mediated tetracycline resistance in Escherichia coli containing more than one copy of the transposon.

In Escherichia coli a single copy of Tn10 confers high-level resistance to tetracycline. Resistance itself results from expression of three distinct mechanisms which normally act together (Shales et al., 1980). In cells containing two copies of Tn10, the level of resistance to tetracycline was reduced. This was not due to overproduction of the repressor which controls the resistance genes, beca...

Integrative Binding Sites within Intracellular Termini of TRPV1 Receptor

TRPV1 is a nonselective cation channel that integrates wide range of painful stimuli. It has been shown that its activity could be modulated by intracellular ligands PIP2 or calmodulin (CaM). The detailed localization and description of PIP2 interaction sites remain unclear. Here, we used synthesized peptides and purified fusion proteins of intracellular regions of TRPV1 expressed in E.coli in ...

ERDC TN-SWWRP-06-10, Sediment-tracing technology: An overview

Environmental and economic problems in water bodies can result from the lack of understanding of these processes. For example, sediment moving through a watershed transports nutrients and pollutants that can damage aquatic habitat and reduce soil productivity in agricultural areas. In addition, sediment movement and deposition in streams can increase the cost to maintain navigation and interfer...